FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49026369
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG870020.17745960342280295No Hit
GCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTG492370.1004296279824435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG44850.029.1401486
CGGTATA70950.023.93977220
GCCGGCT197750.023.17871
GTTCGAC57100.022.9677055
CGACGAG56400.022.8535698
TCGACGA61000.021.8677147
TCACGCT262050.019.4000478
CCGGCTT278700.019.2747862
TTCACGC270350.019.02917
TCGCACC123650.018.95621735
CGGCTTC298350.018.3217033
TGTCGCA127200.018.32106433
AACGCTA269800.018.3107523
GTATAGA101450.018.29484422
ACGCTAC272500.018.14572124
CGCACCC130200.017.95075236
AGTTCGA74250.017.935394
TATAGAG104050.017.77237323
GACGAGG91000.017.6743379
ATGACGG93700.017.59805316
Produced by FastQC (version 0.11.5)