FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53912109
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG175250.013.04268838
GGGAGGT284100.012.6195843
AGCTTAG183200.012.44839345
GGCATAG184100.012.31732635
GGAGGTC296700.012.045564
GGTCACT307000.011.36210257
GTCACTC318400.011.0613238
GTTGTCG22400.011.04844626
GAGCTTA209750.010.93846244
ACTCTCC324650.010.81362711
TGGCATA212250.010.73685534
GAGGTCA368850.010.0368825
CACTCTC348650.09.98501410
TCACTCT368950.09.783299
CATAGGG239650.09.66915237
AGGTCAC379400.09.5970226
GCATAGG251650.09.19001836
TGGGAGG272800.09.1745062
TCAGACG92900.08.988362
GCTCTGT397950.08.72345722
Produced by FastQC (version 0.11.5)