FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51099755
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1621980.3174144377013158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT301900.026.95209345
AGACCGC357200.023.0713024
CAGACCG365850.022.7041993
GACCGCG368950.022.3060515
ACCGCGT376850.021.8713386
AGAACGA380500.021.6506444
CCGCGTT390850.021.0715397
CGCGTTC395700.020.8398428
TCAGACC420750.019.9503212
AATACGG418200.019.68818932
TACGGAG418900.019.62890834
ATACGGA421950.019.51902433
GCGTTCT428250.019.282139
AAGAACG430550.019.11815343
CTCAGAC466100.019.0714261
CAATACG439100.018.8689531
ACGGAGA448300.018.32656735
CGGAGAG454500.018.0468636
CCCAATA463250.017.88885929
CCCCAAT482500.017.2164728
Produced by FastQC (version 0.11.5)