FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51099755
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33000.017.11320726
AGCTTAG158200.015.84336145
ATAGGGG172600.014.45624638
GGCATAG174350.014.23371835
TGTCGTT42400.013.90288528
GAGCTTA182800.013.67426344
GGGAGGT266250.013.0582463
TGGCATA192550.012.935060534
ATATAGC50750.012.8589096
CATAGGG197500.012.71340637
GTCACTC273200.011.9763658
GCTAAGT49250.011.96914914
GGTCACT271200.011.9651387
GCATAGG211000.011.82534736
GTCGTTC50300.011.7192529
GGAGGTC298250.011.2648494
ACTCTCC297400.011.23453111
ATAGCTC73050.010.84144739
TCGTTCC54900.010.7782730
ACCCGGC82100.010.7444935
Produced by FastQC (version 0.11.5)