FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48357859
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT656810.135822803900396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA4300.014.12743837
ACGCAAG67400.014.08753645
AGCTTAG136150.013.86521945
GACGCAA70100.013.5129544
GGCATAG147950.012.819571535
ATAGGGG152150.012.68805638
ATGATCG175900.012.17737145
GTCACTC213500.011.8446368
TTGGTAG81800.011.6953573
GAGCTTA166250.011.51738644
TGGCATA170350.011.29209534
TATCCTA63150.011.1520525
TCACTCT232700.011.0413599
CACACTC110450.010.93842124
TAAGTCG89450.010.76546720
CACGAAG119600.010.76007834
GCCACGA119850.010.75643832
CCACGAA118850.010.67657233
ATAGCTC47150.010.59381539
TAGGCTA64600.010.58744510
Produced by FastQC (version 0.11.5)