FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56371483
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1676110.29733296177430707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT315200.025.69024345
AGAACGA384600.021.17709244
AGACCGC384150.021.1535684
CAGACCG391150.020.861233
GACCGCG404500.020.0559985
ACCGCGT412500.019.7542486
CGCGTTC423550.019.2770678
CCGCGTT424200.019.2082947
GCGTTCT448600.018.1895549
AAGAACG450850.018.090643
TCAGACC461100.017.9793642
ATACGGA453600.017.96016133
AATACGG453150.017.95214532
TACGGAG452550.017.91384534
CTCAGAC490850.017.673541
CAATACG482850.016.98658431
ACGGAGA480100.016.8909535
CCCAATA502750.016.35020629
CGGAGAG496500.016.34209636
CCCCAAT530750.015.60588828
Produced by FastQC (version 0.11.5)