FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47007936
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG129850.015.00472445
ATAGGGG135800.014.62936538
GGCATAG134600.014.50916535
GTCACTC211750.013.323238
GGTCACT211900.013.2604887
GGGAGGT246400.012.7025843
CATAGGG157700.012.64064737
GAGCTTA159400.012.46298644
ACTCTCC233250.012.4328811
TGGCATA162900.012.15428434
GCATAGG165750.011.94528236
AGGTCAC259900.011.4519956
GAGGTCA269100.011.1454265
CACTCTC255100.011.08570910
TCACTCT257200.011.0652329
ATAGCTC66500.010.96169539
ACCCGGC73600.010.9442365
GGAGGTC282700.010.5859684
ATCCTAG92950.09.8992326
GCTCTGT294350.09.860929522
Produced by FastQC (version 0.11.5)