FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44576958
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT970890.21780086474272203No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG770820.17291893269163858No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAG543350.12189032728523108No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC531470.11922527329029495No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG459070.10298369843900071No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC456240.10234884130047636No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC452770.10157041222956488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT208750.022.13823945
AGACCGC216950.021.2915944
CAGACCG231100.020.0862833
ACCGCGT238100.019.4187626
GACCGCG237300.019.3897615
CCGCGTT245000.018.8443167
CGCGTTC247900.018.6874038
AGAACGA255650.018.24410644
TCAGACC268300.017.762562
GCGTTCT261600.017.682949
CTCAGAC309250.016.837451
AATACGG277300.016.72234732
CGTTACG13200.016.7056421
ATACGGA278100.016.66615133
GTCCGTT26100.016.122181
TACGGAG285850.016.11984634
CAATACG291450.016.02626831
AAGAACG291350.015.97771243
ACGGAGA295100.015.59931535
CGGAGAG301000.015.29354836
Produced by FastQC (version 0.11.5)