FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44576958
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG516940.11596574176281838No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCC498350.1117954257892609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG173550.014.80801645
GGCATAG185050.013.833796535
GGGAGGT265300.013.8186483
ATAGGGG188700.013.60272338
GGAGGTC271200.012.9859594
GAGCTTA200400.012.82374844
TGGCATA201200.012.75677834
GTTGTCG20850.012.62287426
GTCAGGA211950.012.0914861
CATAGGG221800.011.7046237
GCATAGG232700.011.17521836
TGGGAGG262500.010.5861172
TATCCTA88700.010.5847445
ACTCTCC333000.010.58154911
TGTGCAA179550.010.50149412
GGTTCTT198200.010.4759336
ATCCTAG99250.010.2931796
TCTCCCC332400.010.27548413
TGTCCAA351100.010.06523326
ACCCGGC52050.010.0354125
Produced by FastQC (version 0.11.5)