FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48728697
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2653220.5444881893722707No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA767070.15741648088804838No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG626740.12861825548095407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT424900.030.5779845
AGAACGA518950.025.0439944
ACCGCGT546400.024.134456
GACCGCG546950.024.1189545
AGACCGC549650.024.0782574
CGCGTTC552500.023.8801828
CCGCGTT552500.023.8639167
CAGACCG568150.023.3471763
TCAGACC596400.022.483212
GCGTTCT600750.022.029639
AAGAACG596100.021.778643
CTCAGAC649400.021.4121651
AATACGG613950.021.2755332
ATACGGA619050.021.07097833
TACGGAG619050.021.04155534
CAATACG635350.020.58413131
ACGGAGA645900.020.1981335
CGGAGAG643700.020.14831436
CGTTCTC664100.020.10096210
CCCAATA672000.019.49885729
Produced by FastQC (version 0.11.5)