FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48728697
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG553700.11362914136612355No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG487890.10012375253949433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG189750.013.89916845
GGGAGGT289100.013.6333283
GGAGGTC291150.013.365224
GGCATAG204350.012.95907135
ATAGGGG204900.012.8481638
AGGGATC153000.012.3971942
TGGCATA219450.012.04686134
GTTGTCG24300.012.03731526
GAGCTTA223200.011.82579344
GGTCACT339050.011.3394277
ACTCTCC350450.011.07457411
GTCACTC357650.010.9075848
CATAGGG248400.010.66151137
TGGGAGG276100.010.3007282
GAGGTCA402150.010.1801815
GCATAGG265500.010.00833636
TAGGGGA373650.09.99663739
CAGGGAT214100.09.935041
CACTCTC397200.09.81622410
GCTCTGT395250.09.70658522
Produced by FastQC (version 0.11.5)