FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50248692
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1049270.20881538568207908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT196750.025.40948945
AGACCGC235200.021.46054
AGAACGA239600.020.92154544
CAGACCG242250.020.9009783
ATACGGA270050.018.49584433
TACGGAG271800.018.38503534
AATACGG275450.018.20675832
GACCGCG280800.017.999455
AAGAACG279850.017.93656243
TTCGACG16600.017.890846
CCGCGTT285100.017.7325337
CGCGTTC286500.017.6615898
CAATACG290800.017.38497231
ACCGCGT292050.017.3490686
TCAGACC304450.017.0135062
GCGTTCT299300.016.9212999
CTCAGAC327500.016.753841
ACGGAGA305150.016.47894735
CCCAATA318100.015.99196529
CGGAGAG317000.015.79906136
Produced by FastQC (version 0.11.5)