FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50248692
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG22350.011.57640726
ATAGGGG101250.010.13219238
GGCATAG97550.010.03059435
AGCTTAG98900.010.01004245
ACCCGGC72950.09.5396845
GCATAGG105750.09.21046936
CATAGGG106800.09.18350637
GGTCACT151500.09.1309317
ATAGCTC67550.09.05915239
GTCACTC153950.08.9126458
CACTCGA103900.08.7572285
ACACTCG111000.08.5422724
ATATAGC72350.08.5188946
ACACGGA84850.08.30110116
ACTCTCC172700.08.16763311
TGTCGTT34050.08.12638428
GGGAGGT202250.08.0958393
GAGCTTA128600.08.013267544
TAATCGG36550.07.94907574
CGTAACG14100.07.81877542
Produced by FastQC (version 0.11.5)