FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47382920
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1012240.21362972142704587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT216950.022.41102445
TTCGGCG86100.018.68398544
AGACCGC261400.018.4374034
CAGACCG266400.018.2011533
AGAACGA271300.018.02919644
CGTTACG15750.017.85656521
GACCGCG270500.017.783875
ACCGCGT275000.017.5493586
CCGCGTT280250.017.2045447
TCGGCGC94500.017.02318645
CGCGTTC284600.016.9652968
CCGTACG100000.015.9976244
CGTACGC100900.015.8995285
TTCGACG16300.015.87365156
TCAGACC310000.015.8372182
AATACGG319250.015.25856832
ATACGGA320500.015.23414133
GCGTTCT319400.015.1802529
GTCCGTT31550.015.1321011
TACGGAG322150.015.08625734
Produced by FastQC (version 0.11.5)