FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47382920
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG525860.11098091886274633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181050.014.53373245
GGCATAG198400.013.36272435
ACCCGGC76100.013.1233455
GGTCACT303850.012.8144967
GGGAGGT331450.012.5876493
ATAGGGG210800.012.53251938
GAGCTTA211800.012.35988644
GTCACTC320100.012.3398498
ACTCTCC325750.012.20933111
GAGGTCA336550.012.0902495
TGGCATA222200.011.91098834
GTTGTCG21200.011.6746926
GGAGGTC350100.011.6093074
ATAGCTC85150.011.3377639
CACTCTC353450.011.06772210
TCACTCT362150.011.0316449
CATAGGG241950.010.83616437
AGGTCAC373800.010.8253936
GCATAGG256700.010.336452536
AGCATAG129400.09.98478745
Produced by FastQC (version 0.11.5)