FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49750259
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1646810.33101536215117994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC360150.022.3521274
CAGACCG368650.021.910013
GACCGCG368400.021.7965455
ACCGCGT370400.021.676746
GAACGAT358850.021.30095345
CCGCGTT388950.020.6602787
CGCGTTC389350.020.647278
TCAGACC414200.019.685282
TCGGCGC87600.018.90002345
TTCGGCG88600.018.6869344
GCGTTCT433450.018.6036789
CTCAGAC466650.018.543361
AGAACGA413250.018.53523644
CGTTCTC484600.016.895510
AATACGG459750.016.6101632
ACTCCCC472300.016.5879525
ATACGGA464100.016.4498633
TACGGAG465500.016.34254634
AAGAACG470000.016.3019943
CCTCTCA489600.016.10514319
Produced by FastQC (version 0.11.5)