FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49750259
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA577820.11614411896830527No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG553860.11132806363882447No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG510260.10256429016781601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG179400.013.69447345
GGGAGGT311050.012.6200723
ATAGGGG196600.012.553738
GGCATAG199600.012.33118935
GGAGGTC315500.012.1354094
GAGCTTA212400.011.6197444
ACTCTCC326350.011.46447711
TGGCATA218350.011.29285634
GGTCACT327800.011.2235537
GTCACTC351250.010.8586058
CATAGGG236250.010.45632737
AGGGATC159850.010.3495012
GTTGTCG19750.010.25259226
CACTCTC371950.010.21065410
GCATAGG250500.09.86150636
TCAGACG92300.09.7300682
AGCATAG127950.09.72366545
TCTCCCC396550.09.59955413
TCACTCT408100.09.5934149
TGGGAGG277800.09.5121912
Produced by FastQC (version 0.11.5)