FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51001484
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1784080.349809429074652No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1371200.2688549219469771No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG913600.179132042510763No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA535030.10490479061354371No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG518750.1017127266336015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24150.022.2669246
GACCGCG441800.021.2014355
AGACCGC450750.021.0300714
ACCGCGT448150.020.991156
CAGACCG458500.020.7776553
CCGCGTT471050.019.956287
CGCGTTC477150.019.6964198
TCAGACC511150.019.3021742
CTCAGAC551150.018.8697991
GCGTTCT515950.018.3024449
CCGGCTT488450.018.2046282
GCCGGCT404050.018.1937071
CGTTACG42200.017.27606621
TTCACGC515500.017.2447367
CGTTCTC571200.017.01661910
TCACGCT525850.016.8197258
CGGCTTC546300.016.742273
CCCGGCT86000.016.5358221
GTTCGAC28350.016.3492835
TCGACGA34750.016.0573947
Produced by FastQC (version 0.11.5)