FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51001484
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1280190.25101034315001497No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1037850.2034940787213172No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG716770.14053904784417645No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG518620.1016872371792162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG61700.015.53035426
ATATAGC73150.015.2269096
GGAGGTC279600.013.4376894
GGGAGGT292550.013.0048593
GTCGTTC73600.012.68361229
TATAGCT89250.012.63133057
GCTAAGT80300.012.555366514
GAGGTCA313000.012.2551655
CAATATA104900.012.0390654
TCGTTCC79000.012.01546430
TGTCGTT80750.011.97854628
CGTTCCC82450.011.6218231
TTGTCGT92350.011.301970527
GGTCACT320750.011.2176387
GTCACTC321850.011.1036578
AGGGATC264100.010.9879092
ATAGGGG256900.010.6848338
GGCATAG243300.010.64209635
ACTCTCC337450.010.55033211
ACGCGTT13150.010.4393428
Produced by FastQC (version 0.11.5)