FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42670491
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG541250.12684409935662563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20550.023.2074976
AACGCTA150950.019.50196823
ACGCTAC152500.019.3185124
AGACCGC101700.019.0231444
GCCGGCT155100.018.8202931
CGCTACC159400.018.51050225
TCACGCT168100.018.3072328
CAGACCG107700.018.2557853
GAACGAT111400.017.56920445
AAAACGC170200.017.41195721
GAAAACG170600.017.34208720
CGGTATA35450.017.07224820
CCGGCTT198800.016.985142
TTCACGC183850.016.9713787
AAACGCT180650.016.44439522
GTTCGAC29350.015.8659675
GACCGCG125100.015.4828225
CGGCTTC230800.015.3612023
ACGCTCA202150.015.31260210
ACCGCGT129100.015.177366
Produced by FastQC (version 0.11.5)