FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48767098
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG32750.013.602468524
AGCTTAG111350.012.14730545
GTTGTCG16550.011.96332926
ATAGGGG112550.011.81597338
CGCTCGC38950.011.72616425
GGCATAG119100.011.09017235
GGGAGGT187000.011.0082523
GAGCTTA132650.010.1118144
CATAGGG133000.010.10062937
TGGCATA141150.09.53305834
GCATAGG140850.09.47376736
GGAGGTC231300.09.0555294
GTCACTC223000.08.7364328
GGTCACT234100.08.5623767
GCGTAGG64300.08.43394836
GGCGTAG65750.08.31615335
CGTAGGG65300.08.27034937
CGTCGAT15300.08.23431512
TATCCTA83300.08.0482715
TAGGCTA73850.08.04222610
Produced by FastQC (version 0.11.5)