FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51441835
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2300520.44720799714862425No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA661560.1286034994669222No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG639460.12430738522449675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT406850.026.94155545
AGAACGA494700.022.24289344
AGACCGC555350.020.9398374
CAGACCG571900.020.3889083
ACCGCGT573550.020.302816
GACCGCG573950.020.245575
CGCGTTC585550.019.8997848
CCGCGTT586250.019.8579777
TCAGACC613450.019.081242
AAGAACG578600.019.04514543
GCGTTCT618950.018.8685479
CTCAGAC654400.018.6027531
ATACGGA595400.018.50884233
TACGGAG594550.018.49544534
TTCGACG18600.018.4952436
AATACGG595900.018.4885832
CAATACG613200.017.98754731
ACGGAGA624300.017.63009635
CGGAGAG641550.017.17010536
CCCAATA647300.017.08785429
Produced by FastQC (version 0.11.5)