FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48017052
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT603300.12564286537207656No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG496520.10340493206455073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC136450.021.9473514
GACCGCG136300.021.8724615
ACCGCGT142200.021.0577626
CAGACCG152400.019.7832623
CGCGTTC153800.019.469598
CCGCGTT154150.019.3963767
TCAGACC190000.016.2004432
CGTACGC41200.016.1102565
CCCGTAT35700.016.0979021
GTTCCTA89650.015.98534515
CGTTACG29250.015.6907921
GAACGAT191150.015.59497945
GCCGGCT178300.015.4334021
CCGTACG43700.015.3945634
GCGTTCT198400.015.1495569
CTCAGAC241650.014.3998461
CCGGCTT216600.014.2109152
CTCCGGT56300.014.170731
TCACGCT205200.013.9677738
GTCCGTT35350.013.8346331
Produced by FastQC (version 0.11.5)