FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54965273
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1736750.31597223214010056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT315650.026.48105845
AGACCGC371300.022.7120194
CAGACCG373900.022.6257613
GACCGCG374300.022.5600385
ACCGCGT378050.022.3124526
CCGCGTT393900.021.4032067
CGCGTTC394550.021.3850548
AGAACGA391500.021.35170744
TCAGACC432150.019.8519482
AAGAACG435150.019.2049243
GCGTTCT441550.019.1934819
AATACGG433900.019.11693832
TTCGACG28900.018.9186236
TACGGAG441350.018.83503234
ATACGGA443400.018.77840233
CTCAGAC481950.018.7663141
CAATACG460550.018.1317931
ACGGAGA465050.017.8655635
CCCAATA476800.017.55447229
CGGAGAG480150.017.3219136
Produced by FastQC (version 0.11.5)