FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46308174
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT615790.13297652375582764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG28600.026.4335832
GACCGCG149950.021.246435
AGACCGC154050.020.8708294
ACCGCGT152950.020.8444046
CGTCCGC39350.019.49795733
CCGCGTT163600.019.4737287
CAGACCG169650.019.1108173
CGCGTTC170400.018.6437938
TCGTATG43150.017.98901745
TCACGCT221750.017.0558438
TTCACGC225100.016.9119637
GATCGGA46350.016.6162321
TCAGACC204850.016.4091172
AGTCACG48150.016.12183428
ACGCTAC234500.015.64936324
AACGCTA234350.015.62082423
ACGCTCA243500.015.58781610
CACGCTC245850.015.5486369
GCGTTCT208250.015.3956859
CCGGCTT189750.015.2367342
Produced by FastQC (version 0.11.5)