FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46308174
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT32800.022.77428211
TCGTGTA41700.018.39919314
GGTCGCC43000.017.31968543
GTCGTGT43550.017.25594513
CGTCGTG45200.016.5264712
GTTGTCG22150.016.1512526
AGCGTCG48150.015.84104510
TGTAGGG52400.014.856817
GTCGCCG53700.014.24574344
TCTCGGT55150.013.91211136
GTAGGGA56850.013.61471118
AGAGCGT59450.013.3220568
ATCTCGG60550.013.0801535
TGGTCGC70200.012.27558342
ATAGGGG154700.011.70819138
GGCATAG149950.011.64392635
AGTGTAG65850.011.61753428
GATCGGA66200.011.5898251
CGTAACG14000.011.57137342
AGCTTAG154350.011.25359145
Produced by FastQC (version 0.11.5)