FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49139471
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG120200.011.90149445
AGCGCTC78800.011.077329
ATAGGGG130900.010.96805238
GGCATAG131200.010.9085935
ATAACGC73700.010.77865539
CGCTCTC78350.010.62043911
TAACGCC78500.010.20621540
GAGCTTA143450.010.12910244
TGGCATA145800.09.87705434
GTCAGGA173050.09.8201041
ACCCGGC61850.09.6779785
GGGAGGT226450.09.6008973
GGACGTC95000.09.5866319
CGGGACG54450.09.54031717
CATAGGG152250.09.44471637
CAGCGCT92850.09.3797888
GTTGTCG19750.09.34096726
GCAGCGC93400.09.3246687
GCATAGG157250.09.2016536
GGAGGTC223750.09.1220554
Produced by FastQC (version 0.11.5)