FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47082129
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2680740.5693752718786358No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1256780.26693355349330106No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG777680.1651751984282614No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG637460.1353931977035278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT494850.026.8112161
CCGGCTT596250.024.6071362
TCACGCT635100.022.9920758
CGGCTTC659200.022.7111723
TTCACGC649700.022.6312127
ACGCTCA701500.021.22625510
AGACCGC285850.020.5641064
CACGCTC712400.020.5446559
CAGACCG292850.020.1647453
GGCTTCA770250.019.7493174
GACCGCG302000.019.4271585
GCTTCAC785500.019.345855
ACCGCGT306600.019.179716
AACGCTA766650.018.76557523
ACGCTAC769850.018.68220924
AAAACGC781250.018.471621
GTTCCTA87950.018.44200115
GAAAACG786100.018.34487720
CGCTCAG814850.018.32050111
CGCTACC785950.018.26875925
Produced by FastQC (version 0.11.5)