FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47082129
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1233050.26189342457304765No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1068650.2269757172620635No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1032840.2193698589968181No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG655360.13919506486208388No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC499430.1060763416199807No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA475940.10108718745492583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT429200.016.6954483
GGTCACT454200.015.4418687
GGAGGTC464200.015.3932934
AGCTTAG326000.015.33687245
GTCACTC470050.014.921178
TGGGAGG359800.014.4187342
GGCATAG349550.014.31718935
ACTCTCC495900.014.24319411
GTTGTCG28900.014.16656926
ATAGGGG362500.013.93123338
CTGGGAG398400.013.8733571
TCACTCT518050.013.7080439
TGGCATA369400.013.63243234
GAGGTCA531850.013.5923445
CACTCTC519750.013.54631710
GAGCTTA370450.013.5209244
AGGGATC302200.013.0203992
TCTCCCC586050.012.1318513
GCTCTGT588550.012.01201222
CAGGGAT346400.011.9036551
Produced by FastQC (version 0.11.5)