FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49493704
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24250.013.82543326
AGCTTAG161650.012.67754345
GGGAGGT296700.012.0702373
ATAGGGG174200.011.94424538
GGAGGTC281750.011.9111864
GGCATAG170750.011.85568635
GAGCTTA181950.011.324512544
GTCAGGA196400.011.2396551
TGGCATA183050.011.16917334
ACCCGGC79050.011.0817455
TGTCGTT32600.010.83588228
ATAGCTC80250.010.20283939
GGTCACT335650.09.9058687
ACTCTCC344900.09.76623311
TCACCCG98450.09.6774483
AGGGATC158100.09.6449222
CATAGGG217350.09.49009237
GTCACTC357750.09.4573698
GTCGTTC37750.09.35670929
ATATAGC47050.09.0366396
Produced by FastQC (version 0.11.5)