FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42610587
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG930970.2184832609792491No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT479470.11252367868107518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT234100.023.5469781
CCGGCTT296600.021.1095942
TCACGCT304250.020.534458
TTCACGC306450.020.3356467
AACGCTA296550.020.33064823
ACGCTAC297500.020.26572824
CGCTACC307050.019.62149225
AAAACGC314450.019.25221421
GAAAACG313950.019.18664620
CGGCTTC340650.019.1063563
AAACGCT326900.018.54681422
ACGCTCA337700.018.50046510
CACGCTC373000.016.7496179
AGACCGC171400.016.144784
CAGACCG176350.015.831943
CGTGGTT380850.015.74287841
CTACCTC398750.015.40252927
GTTTTCG339050.015.30153345
TTCGACG17150.015.21709256
CGTTACG15400.015.19821721
Produced by FastQC (version 0.11.5)