FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45988367
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC731380.15903587096275892No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG675500.14688497201911951No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT465470.10121472675905191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT175300.020.1871681
CCGGCTT202300.019.2810632
ACCGCGT128700.018.4570876
GACCGCG129050.018.4418725
TTCACGC206700.018.3264927
TCACGCT206250.018.1701628
AACGCTA202400.017.71581823
ACGCTAC202800.017.66978524
AGACCGC134350.017.6306364
GTTCCTA87050.017.31347715
CCGCGTT138600.017.1711837
CGCTACC209400.017.11285625
CGCGTTC140400.016.9670628
CGGCTTC238450.016.7730393
GTCCGTT33350.016.6970181
AAAACGC217700.016.6875621
CACGCTC225950.016.6158189
CTCGGTT86450.016.4317461
GAAAACG225500.016.08039720
CAGACCG148850.016.0491833
Produced by FastQC (version 0.11.5)