FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51536018
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1149480.2230440077850019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC229150.023.9682124
GACCGCG242150.022.7093375
CAGACCG243300.022.6847443
ACCGCGT249500.022.0764166
CGCGTTC262800.020.976268
CCGCGTT263450.020.9159647
TCAGACC290050.019.2921832
GCGTTCT292450.018.9706949
CTCAGAC336650.018.2174531
TTCGGCG93650.016.698444
CCGTACG96900.015.9289844
GTCCGTT38450.015.5755881
TCGGCGC100350.015.51550545
CGTACGC100700.015.506645
CGTTCTC376500.015.05237310
GAACGAT366850.015.0202645
GTTAGTT139000.014.71454744
CCCCGCA186200.014.61150735
TTCGCGC19550.014.3863487
CGTTACG13650.014.33900821
Produced by FastQC (version 0.11.5)