FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48115772
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG921820.19158374929534538No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA808440.16801975036376846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG115000.013.96018545
ATAGGGG120050.013.51218838
GGCATAG126350.012.874063535
CATAGGG136550.011.94535137
GCATAGG141250.011.45230836
GAGCTTA144750.011.12111844
GTCACTC193800.010.9565878
TGGCATA149000.010.83928634
ACTCTCC199550.010.69431611
GTTGTCG20300.010.53598126
AGGGATC206050.010.456032
ATAGCTC63250.010.38665839
ATTTCTA473250.010.3655912
TAGGCTA87050.010.07605410
GGTCACT209600.010.0344737
TATCCTA91300.09.9834645
CATTTCT516350.09.8774131
CAGGGAT241750.09.8490021
GGGAGGT231350.09.76775553
ATCCTAG108100.09.5719336
Produced by FastQC (version 0.11.5)