FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53504744
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT724800.1354646234733877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT150150.024.9854945
AGACCGC194150.019.6545454
CAGACCG200800.019.1381133
AGAACGA196750.019.10200544
AAGAACG212450.017.56315843
CGCGTTC218000.017.4309448
CCGCGTT219850.017.26387
ATACGGA216000.017.25906833
AATACGG218150.017.15084832
GACCGCG222500.017.1199115
TACGGAG218500.017.09259634
ACCGCGT225800.016.7999576
CAATACG231950.016.29534131
TTCGACG20000.016.1997016
TCAGACC252150.015.5271442
ACGGAGA250350.015.01729235
CCCAATA261500.014.5831429
GCGTTCT262700.014.5163499
CTCAGAC297600.014.4965951
CGTACGC43550.014.3625375
Produced by FastQC (version 0.11.5)