FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53504744
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG90350.012.37751138
GGCATAG100550.010.82971935
CATAGGG104650.010.64264437
AGCTTAG104600.010.49464245
GCATAGG110000.010.08383236
GTTGTCG19350.09.53348926
TAATCGG41950.09.0669144
CGTAACG17400.08.92271942
GAGCTTA126750.08.91081344
CACTCGA111250.08.81963355
ACACTCG115800.08.7265484
GGGAGGT179700.08.6545823
TGGCATA129550.08.63123334
GTAACGA42000.08.30329718
ATAATCG46650.08.20187953
ACTCTCC190450.08.04593611
ATCGGTT49100.08.0202016
AATCGGT50700.07.90089135
ACTCGAG134300.07.8917556
ATAACGC31950.07.88774239
Produced by FastQC (version 0.11.5)