FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50081285
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1076870.21502443477638403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT225400.023.51663645
AGACCGC228650.023.0905934
ACCGCGT229400.023.058196
GACCGCG228900.022.9769335
CGCGTTC235750.022.4747228
CCGCGTT237150.022.3331157
CAGACCG247850.021.5379773
AGAACGA264500.020.0232544
TCAGACC275800.019.6082632
ATACGGA283450.018.7091633
AATACGG283800.018.6623432
TACGGAG282400.018.63518134
GCGTTCT289200.018.4609729
TTCGGCG72500.018.1539744
CAATACG306400.017.38128731
TCGGCGC76000.017.31793445
AAGAACG307550.017.28629143
CTCAGAC343350.016.999261
ACGGAGA311600.016.93203735
CGTACGC80900.016.3849815
Produced by FastQC (version 0.11.5)