FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50081285
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG123400.012.30748945
GGCATAG125100.011.68744435
ATAGGGG131700.011.51309138
CATAGGG143950.010.3146737
GGGAGGT233950.010.3027863
GAGCTTA151100.010.21509144
GGAGGTC230350.09.8671834
ACCCGGC77200.09.8236445
ACTCTCC225400.09.8081211
GCATAGG152850.09.77218636
GTCACTC231800.09.6716988
GGTCACT221350.09.6678967
TGGCATA155400.09.62578934
GTTGTCG22050.09.59119526
GAGGTCA258800.09.0607365
CGTAGGG69550.08.92760637
ATAGCTC81100.08.84915839
GCGTAGG71100.08.732336
GGCGTAG71500.08.68293535
TAGGGGA245300.08.65778139
Produced by FastQC (version 0.11.5)