FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46090328
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT862050.18703490242030824No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG686850.14902258886072584No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA504840.10953274188024871No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG482290.10464017526627278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC219200.019.6564374
CAGACCG227100.019.022363
GAACGAT225600.019.0088245
ATTACGC16850.017.3586047
CGTTACG20850.016.94366521
GACCGCG256100.016.885755
GACGTTA16050.016.82203517
GCCGGCT234100.016.601781
TCACGCT251800.016.3607798
TTCACGC258050.016.2086527
CCGGCTT274700.016.1602752
ACCGCGT270000.016.0912536
GTCCGTT34300.016.0744321
TTCGACG16200.015.9718116
CTACACG154400.015.92737645
AGAACGA274100.015.75205744
TTACGCA19900.015.7156778
GCGTTCT279200.015.5690849
AATACGG277150.015.52188232
CCGCGTT280100.015.4949627
Produced by FastQC (version 0.11.5)