FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50006700
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG626760.12533520508251894No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT588790.11774222254217935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT157500.019.4341166
GACCGCG158550.019.2692725
AGACCGC164600.018.5883714
CCGCGTT170950.017.889997
GTTCCTA106400.017.81669215
CAGACCG175250.017.7285333
CGCGTTC173300.017.7122768
CCGGCTT228550.016.904782
TTCGACG26900.016.8883136
CCTACTC115950.016.4462618
GCCGGCT209400.016.0758841
GCACTAC103550.015.8335925
TCACGCT224000.015.6609078
TTCACGC229350.015.442667
CCCAGAT218150.015.3174641
ACGCGTC18550.015.29796240
AGTTACC125550.015.07215834
CGGCTTC266100.014.8654673
TCAGACC214700.014.6080592
GTCCGTT38200.014.03830051
Produced by FastQC (version 0.11.5)