FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51192894
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG917430.1792104193210878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT248100.021.7891162
GCCGGCT216250.021.3474371
TTCACGC254200.020.2578357
TCACGCT251550.020.2566248
AACGCTA253950.019.48619523
ACGCTAC256500.019.35437824
CGGCTTC293000.018.8800683
CGCTACC265350.018.78635225
ACGCTCA285750.018.30458510
CACGCTC285200.018.1821259
AAAACGC275850.018.15875621
GAAAACG282250.017.73617620
GTCCGTT52750.017.6650181
GCACTAC86400.017.1656225
AAACGCT292300.017.12945722
TTGCGTC98150.015.2209243
CCCCCCT46800.015.0534031
CCCCCGT38450.014.6931061
CTCCGGT74850.014.674531
CTCCGAT54800.014.2933781
Produced by FastQC (version 0.11.5)