FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51192894
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG666720.13023682544690676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG243200.013.3784445
GTTGTCG27700.012.99633926
AGCGCTC93450.012.8089519
GGGAGGT351950.012.6644533
GGAGGTC362100.012.2286794
ATAGGGG288450.011.38064538
GAGCTTA293350.011.15267644
GGCATAG288600.011.11743135
CACCCGG105450.010.9672821
GCAGCGC110900.010.9152077
CGCTCTC103400.010.83651411
CAGCGCT115200.010.46871958
TGGCATA314250.010.31739234
GCGTTCG23900.010.26145615
ACTCTCC434350.010.19968411
TGGGAGG349850.09.7949122
CACTCTC453800.09.69310910
CCGCGTT26250.09.68566613
GTCACTC464450.09.6064968
GGTCACT458800.09.5531547
Produced by FastQC (version 0.11.5)