FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54966855
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2305950.41951645223289563No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA569540.10361516954171746No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG553700.10073343290242819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT351900.031.103645
AGAACGA426100.025.72953644
AGACCGC466050.023.526434
ACCGCGT467150.023.415626
GACCGCG470000.023.2568975
CCGCGTT472700.023.1882927
CGCGTTC476300.023.0035828
CAGACCG478700.022.9282283
AAGAACG496550.022.11077343
AATACGG505850.021.64278832
ATACGGA510450.021.43885833
TCAGACC519550.021.436572
TACGGAG515050.021.19919834
CAATACG526600.020.8330331
GCGTTCT533950.020.6126089
ACGGAGA539650.020.2910535
CTCAGAC579150.020.0371171
CGGAGAG554300.019.70194436
CCCAATA565300.019.49437929
TTCGGCG65750.018.71710444
Produced by FastQC (version 0.11.5)