FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47006155
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG476100.10128460836671282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20450.014.85274926
GGGAGGT217150.014.6797173
AGCTTAG170250.014.2372345
GGCATAG174150.013.76069135
ATAGGGG179300.013.67990138
GGAGGTC235650.013.36496454
GAGCTTA194650.012.55660844
TGGCATA204650.011.79805434
TGGGAGG224000.011.5694332
CATAGGG211750.011.3602637
GCATAGG218800.011.035274536
CACCGAA204600.010.14196745
CTGGGAG301900.09.8701271
CGTGGCG95500.09.6858171
GCACCGA213350.09.67327744
GTAACGA38750.09.63737718
AGGGGAG326450.09.43019240
TGTCGTT32950.09.35527528
GCTCTGT328550.09.31291922
CACTCTC334850.09.27895610
Produced by FastQC (version 0.11.5)