FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44917914
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG856850.19075908110959916No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA745640.16600058497818931No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT638910.1422394637471366No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG497140.11067744597400495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC144900.022.9504814
GACCGCG148100.022.2720345
ACCGCGT149450.022.1555376
CAGACCG152850.021.933673
CCGCGTT162150.020.3647677
CGCGTTC166050.019.954248
GCCGGCT262950.019.4027041
CCGGCTT291500.019.1452622
GTTCCTA95200.017.98123615
TTCGACG16450.017.9133056
GCGTTCT188100.017.6151079
TTCACGC313650.017.5492697
CGTTACG38150.017.51351721
CCGTACG31100.017.4594484
TCGTTAC26850.017.3418944
TCACGCT317650.017.2999958
TCAGACC198800.017.260662
CGTACGC32550.017.2354345
CGGCTTC352000.016.4243353
CTCAGAC252000.016.0838641
Produced by FastQC (version 0.11.5)