FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44917914
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA575190.12805358681616424No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG529190.11781268382142591No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG471330.10493140888065283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG36550.016.05982226
GGAGGTC213950.014.4525234
GGGAGGT220900.014.2853973
ATATAGC50700.013.98259456
CGTAACG16050.013.30789642
GGCATAG185050.012.71778935
ATAGGGG196550.012.60293538
GTCGTTC49150.011.76230629
TGGCATA203400.011.64777734
GAGCTTA205250.011.52257544
GGTCACT264400.011.3804527
AGCTTAG211300.011.27845845
GCTAAGT55550.011.2620614
TGTCGTT51150.011.257874528
AGGGATC166950.011.1035612
GTCACTC276050.010.9813648
TGGGAGG241050.010.7177892
TATAGCT68600.010.6950557
AATCGGT102500.010.2358975
CATAGGG239050.010.14570137
Produced by FastQC (version 0.11.5)