FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53337350
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG602590.11297711641092031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG48000.027.94059432
TTCGACG39550.024.9138816
TCGTATG60250.022.3291545
CGTCCGC60000.022.2782433
CGACGAG52250.019.2018388
GTCCGCA73100.018.7782434
CGGTATA71650.018.71217520
TCGACGA55350.018.5734587
GCCGGCT183100.017.5895671
TCACGCT203950.016.7984968
CGTTACG22800.016.6742421
CCGCACA81250.016.64844936
AACGCTA207450.016.08170723
CGTCTGA85050.016.08138716
ACGCTAC209900.015.93789724
ACACGTC87950.015.85806113
AGACCGC60500.015.8404674
GTTCGAC63800.015.5853075
CGCACAT80050.015.57654537
CCCGTAT38000.015.4799011
Produced by FastQC (version 0.11.5)