FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52119965
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1010010.19378562514383882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34750.023.6925356
GAACGAT233900.022.17018545
AGACCGC242950.021.5829834
CAGACCG246900.021.3196873
GACCGCG262100.019.8000685
CGGTATA53000.019.73413820
CCGCGTT266800.019.4250227
ACCGCGT267300.019.423266
CGCGTTC269900.019.2007128
CGACGAG43600.018.5202038
GTTCGAC43650.018.3979765
AGAACGA290100.017.9462244
TCAGACC301650.017.912022
AATACGG303900.016.95006432
ATACGGA303950.016.88802133
TACGGAG304150.016.77361734
GCGTTCT311700.016.7136619
CTCAGAC344400.016.660051
TCGACGA49850.016.5602057
AAGAACG315600.016.5184243
Produced by FastQC (version 0.11.5)