FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52119965
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG132250.011.28079638
AGCTTAG134600.011.21410345
GGCATAG141600.010.56747235
GGTCACT190800.010.47020157
CATAGGG145100.010.23530937
GTCACTC196800.010.094248
GGGAGGT227600.09.9286973
GCATAGG151250.09.80425236
GAGCTTA156550.09.68493744
ACTCTCC211900.09.68470111
CACTCGA112900.09.6811725
TGGCATA160650.09.39832234
ACACTCG121150.09.2816854
GTTGTCG23500.08.81222426
GAGGTCA248500.08.78776555
GGAGGTC248400.08.7187644
AACTCTA100650.08.67171645
CACTCTC239600.08.45256410
ACTCGAG140300.08.4010826
AGGTCAC260600.08.2948896
Produced by FastQC (version 0.11.5)