FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40753881
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG603960.14819692877839047No Hit
GCCAGACTCAGAAAAAAGGTGAAATTTAAAACGAACGAGAAGGGGAAGGGG590250.1448328319945774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589060.14454083526425374No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA479420.11763787600989462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG6200.033.0754551
CGCGTGA9550.031.1477491
CGCGTCG6000.027.7930721
TTCGACG16100.024.3159376
GTTCGAC18900.021.9045495
CAGACCG95150.021.8506243
AGACCGC94350.021.7741534
GCCGGCT172950.021.2254351
GACCGCG98100.020.7796135
CGGTATA28350.020.71553420
CCGGCTT216350.020.4176452
TCGTTAC19650.019.80758344
ACCGCGT103800.019.6814086
CGCTGAG155350.019.2638441
TCGACGA20800.019.1459797
TTCACGC231050.018.4044767
GACGGTA34000.017.8002518
CCCGGCT53150.017.7226681
TCACGCT240750.017.5975288
CCGCGTT116300.017.5853827
Produced by FastQC (version 0.11.5)